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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been observed in several tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a essential function in advertising cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two additional splice variants have been noted but in-depth nucleotide sequence data just isn't still accessible.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to be sure the absolute best outcomes are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be certain the very best effects are...

Antibodies sure to the blocking peptide no more bind towards the epitope about the focus on protein. By comparing the staining in the blocked antibody versus the antibody alone, one can see which staining is certain.

Goal Info Cyclin E belongs to the really conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a fancy with and capabilities as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells development get more info by way of S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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